Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175955_2w93 AATAATACACTTGAGGAATGGTTCTGTGAATG(+AGGACA)GTACCTTTCAATATTTTGGTTATTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613175954_0eq0 ATATTAACAAACAATAACCAAAATATTGAAAG(+AGGACA)GTACCATTCACAGAACCATTCCTCAAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240613175953_o4er CTTACCATCACAGTAAGACATAAGTGGGTGAG(+AGGACA)GATCCCTTCTGTCATTGATTCTTTCTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240613175951_019o CACAGTTTACAGAAAGAATCAATGACAGAAGG(+AGGACA)GATCCTCACCCACTTATGTCTTACTGTGATG NGG 10 8 17 8 24 0 100 5 10
    240613175950_s6sx TCAAAGGCTCAATGCTGCCCTAAATTAAACAG(+AGGACA)GTACCATGGGCAGTCTAAGTGGGTATTGTGC NGG 10 8 17 8 24 0 100 5 10
    240613175947_bsqi CTCAGGCACAATACCCACTTAGACTGCCCATG(+AGGACA)GTACCTGTTTAATTTAGGGCAGCATTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613175946_lkb8 TGTGGGAGCAGGTTTCCTGTAGAGCGTTCCAG(+AGGACA)GTACCACAAGATACAGGGCTCCTATTCAACC NGG 10 8 17 8 24 0 100 5 10
    240613175944_vklj TTGGGGGTTGAATAGGAGCCCTGTATCTTGTG(+AGGACA)GTACCTGGAACGCTCTACAGGAAACCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613175943_8pv1 AGTTTGACTATAAGAATTTCACTTCTCAACAG(+AGGACA)GTACCTGAACAGTACAATATTAACGATATTG NGG 10 8 17 8 24 0 100 5 10
    240613175942_7kpj AAGTACAATATCGTTAATATTGTACTGTTCAG(+AGGACA)GTACCTGTTGAGAAGTGAAATTCTTATAGTC NGG 10 8 17 8 24 0 100 5 10