Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180109_q2x0 TCCACAACTAAATTGACAAATTATTATTTTTG(+AGGACA)GTACCTCACGCCTTTCATCTGAATTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613180107_kxe0 GCACTATGTTGACCAAGCCTGCCAGCCCTATG(+AGGACA)GTACCTTTCTTTAAATGGCCGAATCCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240613180105_5nz4 TACTAACCAGGGATTCGGCCATTTAAAGAAAG(+AGGACA)GTACCATAGGGCTGGCAGGCTTGGTCAACAT NGG 10 8 17 8 24 0 100 5 10
    240613180104_d9lm TGCAGCAAGTCATCTAATGCATGTGCACTTAG(+AGGACA)GATCCAAGGACAAGAAAAGAACAGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613180102_b5n9 TGTTTCTCTCTCCTGTTCTTTTCTTGTCCTTG(+AGGACA)GATCCTAAGTGCACATGCATTAGATGACTTG NGG 10 8 17 8 24 0 100 5 10
    240613180101_zldy CCCCAGTGAGGAGTTAGGGAATGGTTGTGGTG(+AGGACA)GATCCCCTGCCTAGAATCTCCCAGTGAGGCG NGG 10 8 17 8 24 0 100 5 10
    240613180059_g69b CTGAGCGCCTCACTGGGAGATTCTAGGCAGGG(+AGGACA)GATCCACCACAACCATTCCCTAACTCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240613180057_5ap6 GGGGATGCTGCGGACAAGGCATCCAGGATGTG(+AGGACA)GATCCCCAAGGAGCCTTCAGAAAAGATGCCC NGG 10 8 17 8 24 0 100 5 10
    240613180055_zp80 GGAGAGGGCATCTTTTCTGAAGGCTCCTTGGG(+AGGACA)GATCCACATCCTGGATGCCTTGTCCGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613180053_y2ja TGCAATTATGGGGATCTGAGTTTGAATCCCTG(+AGGACA)GTACCCATATAAAGACACAGATGCGGTGATG NGG 10 8 17 8 24 0 100 5 10