Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180019_7hli GCCCAGTGCTGTACCTTCAGTTTCATTGCTCG(+AGGACA)GTACCGCTTACTTCCTGTCTTTTAAATAATT NGG 10 8 17 8 24 0 100 5 10
    240613180017_w900 TGGGCACCTCTGCCCTGCCTGTCTGCTGCCCG(+AGGACA)GATCCTCCTCACTTAGCACCTAGGGGTGTCA NGG 10 8 17 8 24 0 100 5 10
    240613180015_fmrt GACCCTGACACCCCTAGGTGCTAAGTGAGGAG(+AGGACA)GATCCGGGCAGCAGACAGGCAGGGCAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240613180014_b267 CTGCTATACCCTGGGAAGGACAACCATCAGTG(+AGGACA)GTACCTTAGGGGCCTGAGGTTCCAGGGGATC NGG 10 8 17 8 24 0 100 5 10
    240613180012_3gwz CTTTAGATCCCCTGGAACCTCAGGCCCCTAAG(+AGGACA)GTACCACTGATGGTTGTCCTTCCCAGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240613180011_27k0 CACATGCGTGGTCAGTGTCAGTTACCAACTCG(+AGGACA)GTACCATTCAAAGAGAAAAGATGGATTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613180009_hoau TGGGAAACAAATCCATCTTTTCTCTTTGAATG(+AGGACA)GTACCGAGTTGGTAACTGACACTGACCACGC NGG 10 8 17 8 24 0 100 5 10
    240613180007_1ot2 CCAGCTCCCTCACAGGGGGCTGGGGGGAGGGG(+AGGACA)GATCCTCAACAAGGACTCCACCCTCACCATG NGG 10 8 17 8 24 0 100 5 10
    240613180006_idvx GTGGGCATGGTGAGGGTGGAGTCCTTGTTGAG(+AGGACA)GATCCCCCTCCCCCCAGCCCCCTGTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613180004_xwv7 GGGCGCGTTTGTTCCTCACCCACGCGCGTGGG(+AGGACA)GATCCCCTCGCCACAGGGAAGCACAAGAACC NGG 10 8 17 8 24 0 100 5 10