Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175729_k2zr CCTCCTTGGAGGATGTACTGTGATCATTCCTG(+AGGACA)GTACCTTAGGGCCAATCCCTAAGTGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613175727_pekk TTAGGAAGCCACACTTAGGGATTGGCCCTAAG(+AGGACA)GTACCAGGAATGATCACAGTACATCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613175725_n1vv CTTGTTTCACCTCATAAGAACCTCACAAGAAG(+AGGACA)GTACCAGCAGGTTCTGCCCCAAACTTCACTT NGG 10 8 17 8 24 0 100 5 10
    240613175724_xsjs TCCAAAAGTGAAGTTTGGGGCAGAACCTGCTG(+AGGACA)GTACCTTCTTGTGAGGTTCTTATGAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240613175723_3oox GTTCACCCTGCCTGCTTGTAAATTCTAGGAAG(+AGGACA)GATCCCGGAGAACAGCATGGTTTTCTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613175720_wzt7 TTTAATTAAAAGAAAACCATGCTGTTCTCCGG(+AGGACA)GATCCTTCCTAGAATTTACAAGCAGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613175719_kw6i ATTCACCATGTCTTATTAATTTCAAAACTAAG(+AGGACA)GATCCATGCAAGGAGTGGTGTTACAGCCATA NGG 10 8 17 8 24 0 100 5 10
    240613175718_v38y TTTTTTATGGCTGTAACACCACTCCTTGCATG(+AGGACA)GATCCTTAGTTTTGAAATTAATAAGACATGG NGG 10 8 17 8 24 0 100 5 10
    240613175717_n086 GTTGAAGACAGTTTAGCTCCCACAGTGCTGCG(+AGGACA)GATCCCAGCACAATCCCAGTGCGGTCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240613175715_kvih AGGGGAAGGAGACCGCACTGGGATTGTGCTGG(+AGGACA)GATCCGCAGCACTGTGGGAGCTAAACTGTCT NGG 10 8 17 8 24 0 100 5 10