Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175626_lj6a CTCCCTAGAGATCTCAAGGCTAAGGTATAAAG(+AGGACA)GTACCCTGCCTCATTAGTGGAAAGATGTATA NGG 10 8 17 8 24 0 100 5 10
    240613175624_ndxs GAGTGTATACATCTTTCCACTAATGAGGCAGG(+AGGACA)GTACCTTTATACCTTAGCCTTGAGATCTCTA NGG 10 8 17 8 24 0 100 5 10
    240613175623_ret0 CAACCCATAGGGTACATATTTTGTCACAAAAG(+AGGACA)GTACCAGATGCATGCCAAACCAAACAACCTG NGG 10 8 17 8 24 0 100 5 10
    240613175621_2zpq TAATCCAGGTTGTTTGGTTTGGCATGCATCTG(+AGGACA)GTACCTTTTGTGACAAAATATGTACCCTATG NGG 10 8 17 8 24 0 100 5 10
    240613175620_zibp TGTACAGCAGCCATCTCTTGAGGTCACACCTG(+AGGACA)GTACCTACATAGGCAAGAAGGCTTCGGGATA NGG 10 8 17 8 24 0 100 5 10
    240613175618_jsvl CTATGTATCCCGAAGCCTTCTTGCCTATGTAG(+AGGACA)GTACCAGGTGTGACCTCAAGAGATGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613175617_zrqp TTCCCTGAGACTTGGTACTCTATGAGGTGGAG(+AGGACA)GTACCCATCCCTGATCTGAACCCTTTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613175615_hlon CTAACCCAGAAAAGGGTTCAGATCAGGGATGG(+AGGACA)GTACCTCCACCTCATAGAGTACCAAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613175613_u8cp CAGACGCAGACGGGGCAGCAAGGACGGCCCTG(+AGGACA)GATCCAACTCTTCAGCTACTGCTCCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613175612_3fxb GGCTTGGCTTGGGAGCAGTAGCTGAAGAGTTG(+AGGACA)GATCCAGGGCCGTCCTTGCTGCCCCGTCTGC NGG 10 8 17 8 24 0 100 5 10