Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175551_s62r GTGCAGTGCAGTGCAGTGCAGTGGGATAGATG(+AGGACA)GTACCCCTCACCACCCCCTCGCCCTGTGAGG NGG 10 8 17 8 24 0 100 5 10
    240613175549_u9s2 ATTATCCTCACAGGGCGAGGGGGTGGTGAGGG(+AGGACA)GTACCATCTATCCCACTGCACTGCACTGCAC NGG 10 8 17 8 24 0 100 5 10
    240613175548_pj7f AATTAAATAACTTTCATGGGGTTCAGGGTTAG(+AGGACA)GATCCCCAGCCTTCCCTCTTGGGGGAAACCG NGG 10 8 17 8 24 0 100 5 10
    240613175546_rfz7 TGCCTCGGTTTCCCCCAAGAGGGAAGGCTGGG(+AGGACA)GATCCTAACCCTGAACCCCATGAAAGTTATT NGG 10 8 17 8 24 0 100 5 10
    240613175544_8nra ATCCCAGTTTGGGATACGTTCTCTCGCACCAG(+AGGACA)GTACCCATCCATGGTGCCTTTGTTTCTTCGG NGG 10 8 17 8 24 0 100 5 10
    240613175543_sb3e ACAGTCCGAAGAAACAAAGGCACCATGGATGG(+AGGACA)GTACCTGGTGCGAGAGAACGTATCCCAAACT NGG 10 8 17 8 24 0 100 5 10
    240613175541_obs8 AGTGCCACCCTCCCAGCTGGCCCTGTGGTTGG(+AGGACA)GATCCTGCCTGACTTCTTCCAGCTATCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613175539_c9rg CCCCAAGGGGATAGCTGGAAGAAGTCAGGCAG(+AGGACA)GATCCCAACCACAGGGCCAGCTGGGAGGGTG NGG 10 8 17 8 24 0 100 5 10
    240613175537_5zbp AAAGCTGACATAGCACATTCTTGGCACTCCGG(+AGGACA)GTACCAAGGAGGCTATTGGGGGCGGAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613175535_cuy8 TTGCATGCTCTCCGCCCCCAATAGCCTCCTTG(+AGGACA)GTACCCGGAGTGCCAAGAATGTGCTATGTCA NGG 10 8 17 8 24 0 100 5 10