Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175442_axzj ATTCTTCAAACATGACTGAGACTGACTCTCAG(+AGGACA)GATCCTGCTCAGCGCAGGTTCACAGAGGAGC NGG 10 8 17 8 24 0 100 5 10
    240613175441_9fz1 TGTCTGCTCCTCTGTGAACCTGCGCTGAGCAG(+AGGACA)GATCCTGAGAGTCAGTCTCAGTCATGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613175440_0790 CCGGAGATTTAGCAGCTCAGTGTTGGTGTGAG(+AGGACA)GTACCTGAAACCCCGTCTAATAGGGAGCGGG NGG 10 8 17 8 24 0 100 5 10
    240613175439_5n6k CCCGACCCGCTCCCTATTAGACGGGGTTTCAG(+AGGACA)GTACCTCACACCAACACTGAGCTGCTAAATC NGG 10 8 17 8 24 0 100 5 10
    240613175437_92my GAGACACCTGCACGCTGGCACACACCCTGAGG(+AGGACA)GATCCCTGGGACTTCGGCTCCGATGAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613175434_i07s CCAAGGTCTCTCATCGGAGCCGAAGTCCCAGG(+AGGACA)GATCCCTCAGGGTGTGTGCCAGCGTGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613175432_tw6w GTGGATGGCACTGGAGCTGTGAGTGAAATGGG(+AGGACA)GTACCAGGGCAGGAGACTGCGCCCACAATGT NGG 10 8 17 8 24 0 100 5 10
    240613175430_m6wy GGAGAACATTGTGGGCGCAGTCTCCTGCCCTG(+AGGACA)GTACCCCATTTCACTCACAGCTCCAGTGCCA NGG 10 8 17 8 24 0 100 5 10
    240613175427_jvrb ATGGGTCATAAGTGGGAGAGCTGGAGGCCCAG(+AGGACA)GTACCACCCTCCCCCTGGGCTATCCCGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613175425_8b29 AGGGGGTGCCGGGATAGCCCAGGGGGAGGGTG(+AGGACA)GTACCTGGGCCTCCAGCTCTCCCACTTATGA NGG 10 8 17 8 24 0 100 5 10