Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175409_55ks AGCATCTTCACTCTTCTGGCCTACCAAGCTTG(+AGGACA)GTACCTATTTCTCATCATAACTTTGGAATGT NGG 10 8 17 8 24 0 100 5 10
    240613175408_iys0 ACCCTACATTCCAAAGTTATGATGAGAAATAG(+AGGACA)GTACCAAGCTTGGTAGGCCAGAAGAGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613175406_rg7i ACATGCCTTTGGGTGTCTGTTTGCCAGGCTGG(+AGGACA)GATCCCGGGCTCCTGAGTGAGCAGTAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613175403_7yn1 ACAGTCCAGCTACTGCTCACTCAGGAGCCCGG(+AGGACA)GATCCCAGCCTGGCAAACAGACACCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613175402_0bi9 AAGATAACCAAGGCTTCCACTGGCAGGGTTTG(+AGGACA)GATCCCAGGCAGGCAAGCAGGCAGGCTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613175359_du8j CCATCCACCAGCCTGCCTGCTTGCCTGCCTGG(+AGGACA)GATCCAAACCCTGCCAGTGGAAGCCTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613175357_0qy9 CTGCCTCAGCCTGTGCTCCAGGTTGGTTCCAG(+AGGACA)GTACCCTAGCGGCCTTGGCAGGTTTTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613175356_778g GATGTGCTGCAAAACCTGCCAAGGCCGCTAGG(+AGGACA)GTACCTGGAACCAACCTGGAGCACAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613175354_n9k4 TAATGAGCTTCCTTTTCTCCAATGTGTATGAG(+AGGACA)GTACCTGTATGATGTGCTCTGATCACACCCA NGG 10 8 17 8 24 0 100 5 10
    240613175353_s68l AAGGGTGGGTGTGATCAGAGCACATCATACAG(+AGGACA)GTACCTCATACACATTGGAGAAAAGGAAGCT NGG 10 8 17 8 24 0 100 5 10