Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175352_o6ky GTTTCTAACAGAAATTACGATTTGAGGGTCAG(+AGGACA)GATCCTGGGAGAGACTCTCCAGCTCCAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613175349_cg6z AATCTGTCCTGGAGCTGGAGAGTCTCTCCCAG(+AGGACA)GATCCTGACCCTCAAATCGTAATTTCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240613175348_10h5 TGAACTTCACCCTGAAAGAGTGAGCTGTGCTG(+AGGACA)GATCCTCCCCGTAAGAAATTCTATAGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240613175346_xhgo AATCACTTCTCTATAGAATTTCTTACGGGGAG(+AGGACA)GATCCAGCACAGCTCACTCTTTCAGGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240613175345_0uzh AAGGATTCCGAAGAATAAGTTACAGCTGTCTG(+AGGACA)GATCCTGCTTGGGAATTTTTAAGGGATATGG NGG 10 8 17 8 24 0 100 5 10
    240613175344_x7dh CTTTCCCATATCCCTTAAAAATTCCCAAGCAG(+AGGACA)GATCCAGACAGCTGTAACTTATTCTTCGGAA NGG 10 8 17 8 24 0 100 5 10
    240613175342_wk2f TTACTTCCTCTTGATTCATTTTTTGAGTGTAG(+AGGACA)GTACCTTATAGTTCTGTCAAAGGTAGACAGA NGG 10 8 17 8 24 0 100 5 10
    240613175341_870w TCAACTCTGTCTACCTTTGACAGAACTATAAG(+AGGACA)GTACCTACACTCAAAAAATGAATCAAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613175339_tp6j AAATCTTTTTAAAAAAGCAAACAAAAAAATGG(+AGGACA)GTACCAGATGAAAGGGGTTCAGGACATGGTT NGG 10 8 17 8 24 0 100 5 10
    240613175338_4r1f GAAGCAACCATGTCCTGAACCCCTTTCATCTG(+AGGACA)GTACCCATTTTTTTGTTTGCTTTTTTAAAAA NGG 10 8 17 8 24 0 100 5 10