Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175657_744h CTGTCTCAGAAGAGCATCTGAACTTGTCCTCG(+AGGACA)GTACCAAGGAATCTCTCCAACAACTGGGTCT NGG 10 8 17 8 24 0 100 5 10
    240613175655_kg5i AATCAAGACCCAGTTGTTGGAGAGATTCCTTG(+AGGACA)GTACCGAGGACAAGTTCAGATGCTCTTCTGA NGG 10 8 17 8 24 0 100 5 10
    240613175653_xh8b TGAGCAATTAGAAAATAAGGCTGAATACAGAG(+AGGACA)GTACCTTTAAAATTACAAAGAGACTTAACAT NGG 10 8 17 8 24 0 100 5 10
    240613175652_fz7h GCCTTATGTTAAGTCTCTTTGTAATTTTAAAG(+AGGACA)GTACCTCTGTATTCAGCCTTATTTTCTAATT NGG 10 8 17 8 24 0 100 5 10
    240613175651_9crl GCATCTGAAAGGCATTACCTAGCAGGAATGAG(+AGGACA)GTACCCTGTGATCACTATTCCTGGTTATCAA NGG 10 8 17 8 24 0 100 5 10
    240613175649_w6an TCAAGTTGATAACCAGGAATAGTGATCACAGG(+AGGACA)GTACCTCATTCCTGCTAGGTAATGCCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613175647_8ovl ATTGTAGTACAACTTTGAATCATCTCAGCAGG(+AGGACA)GATCCTCGATTGTGAAACTGTCTTGATCATC NGG 10 8 17 8 24 0 100 5 10
    240613175646_9k3t ATTAAGATGATCAAGACAGTTTCACAATCGAG(+AGGACA)GATCCCTGCTGAGATGATTCAAAGTTGTACT NGG 10 8 17 8 24 0 100 5 10
    240613175644_35sh CCTAAAGTTCCACAAGGGATGAAGAAGTCCTG(+AGGACA)GATCCCCCTGTGGTACAGGTGAGAAAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613175642_r4m1 GTCTCCTCCTTTTCTCACCTGTACCACAGGGG(+AGGACA)GATCCAGGACTTCTTCATCCCTTGTGGAACT NGG 10 8 17 8 24 0 100 5 10