Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175746_95u1 GGCCAGGAAAGGGTTAATGAGAGCCAAGGTGG(+AGGACA)GTACCTGGAACCATGGCTGGAGGCCTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613175744_3mgw TGCTTTCCCCAGGCCTCCAGCCATGGTTCCAG(+AGGACA)GTACCCACCTTGGCTCTCATTAACCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613175742_sf3k TGGCCTCCCAACCAAGGGAGCCATCATGGTGG(+AGGACA)GTACCAAGAAAGGCAAAGCACTTGGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613175740_lchj AAAAAAAAGCCCCAAGTGCTTTGCCTTTCTTG(+AGGACA)GTACCCACCATGATGGCTCCCTTGGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613175739_t16i TGTGGCCTCAGGCCTCAGTTCTTGGCCAACTG(+AGGACA)GTACCTGAATGTCTTGTTCTCATTTAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613175738_lhkm AAGCTGCCCCTAAATGAGAACAAGACATTCAG(+AGGACA)GTACCAGTTGGCCAAGAACTGAGGCCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240613175736_utaq CAGTCACAGCGTGCCAGGGACTCAAGGAGAGG(+AGGACA)GTACCTGGAAGACTGTATGTCCTGAGGATAA NGG 10 8 17 8 24 0 100 5 10
    240613175734_b7sf AGCCCTTATCCTCAGGACATACAGTCTTCCAG(+AGGACA)GTACCCTCTCCTTGAGTCCCTGGCACGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613175733_mihf CAGTCACAGCGTGCCAGGGACTCAAGGAGAGG(+AGGACA)GTACCTGGAAGACTGTATGTCCTGAGGATAA NGG 10 8 17 8 24 0 100 5 10
    240613175731_vpz5 AGCCCTTATCCTCAGGACATACAGTCTTCCAG(+AGGACA)GTACCCTCTCCTTGAGTCCCTGGCACGCTGT NGG 10 8 17 8 24 0 100 5 10