Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175940_23qw TTGTGGAAGGCAAGGATGTTGGAGAATAGGAG(+AGGACA)GTACCCAAGCCTCCTGCCTGTCTTAAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240613175939_h96v TAGAACTACCTTAAGACAGGCAGGAGGCTTGG(+AGGACA)GTACCTCCTATTCTCCAACATCCTTGCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613175938_0h8o GAAAAATAAATTAAGAAATCAAAACAACACTG(+AGGACA)GATCCATAAGCAATCCGAAGCCGCTTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613175936_e3a6 AGCGACAAGCAAGCGGCTTCGGATTGCTTATG(+AGGACA)GATCCAGTGTTGTTTTGATTTCTTAATTTAT NGG 10 8 17 8 24 0 100 5 10
    240613175935_kbt8 AAGACAGATAGAAACCTGATTTCTCTCACTGG(+AGGACA)GATCCACAAGGTTAAAGGAGGAAAAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240613175933_ylzy GCAAGTCATTTTTTTCCTCCTTTAACCTTGTG(+AGGACA)GATCCCAGTGAGAGAAATCAGGTTTCTATCT NGG 10 8 17 8 24 0 100 5 10
    240613175932_5v15 GACAAGAAAAACAACTGTGATGTGGGCAGCAG(+AGGACA)GTACCTGGGGGTGGAGGGTCTTATTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613175929_cslj CCTGGAGAGGCAATAAGACCCTCCACCCCCAG(+AGGACA)GTACCTGCTGCCCACATCACAGTTGTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613175928_152m CAGGCCAATTATCCTCGGGAAGGAGGGTAAAG(+AGGACA)GATCCCTCACTATCTTTTGAATTTGGACTGA NGG 10 8 17 8 24 0 100 5 10
    240613175926_xrt4 CTAGATCAGTCCAAATTCAAAAGATAGTGAGG(+AGGACA)GATCCTTTACCCTCCTTCCCGAGGATAATTG NGG 10 8 17 8 24 0 100 5 10