Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142703_mbvg CACACCCCATTGTGACCCAGAGCCTACTCCTG(+AAAAAA)GTACCTGCACTTGATGTTCCTTAGATTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606142701_ts9l GGTGTCAGCGTTTGCATGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606142659_34xt GGATGGACCATGTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCACCCCATAATCAGCATGCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606142658_xrhs TTTCAATGGAATCTAAGGAACATCAAGTGCAG(+AAAAAA)GTACCAGGAGTAGGCTCTGGGTCACAATGGG NGG 10 8 17 8 24 0 100 5 10
    240606142655_5m6t CACACCCCATTGTGACCCAGAGCCTACTCCTG(+AAAAAA)GTACCTGCACTTGATGTTCCTTAGATTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606142654_iahy GGTGTCAGCGTTTGCATGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606142651_wjh6 GGATGGACCATGTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCACCCCATAATCAGCATGCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606142650_2gcv ATGTTTATAATTTTATATGAGCTACATTCTAG(+AAAAAA)GTACCCTTAAACAAGTTTGATACATGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240606142648_2453 ACCCATGACCCATGTATCAAACTTGTTTAAGG(+AAAAAA)GTACCTAGAATGTAGCTCATATAAAATTATA NGG 10 8 17 8 24 0 100 5 10
    240606142646_q34o ATTTTGCCATGTGGCTGGTTACCTGTGTTTGG(+AAAAAA)GTACCACACATCAGTCTCCTCATGTCTTACC NGG 10 8 17 8 24 0 100 5 10