Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142554_4w73 AATTTGGAATGAATTTGTCCCAGAGTTCACTG(+AAAAAA)GTACCTATATTGCTTTTTTTTTTTTTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606142553_wdi0 TGTTTTTCTGTTTGTGTTCACTCCTTTTCTAG(+AAAAAA)GATCCAGGCAAAGGTGTAAGCCGAGCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240606142551_0yog TTGAGTTAAAGCTCGGCTTACACCTTTGCCTG(+AAAAAA)GATCCTAGAAAAGGAGTGAACACAAACAGAA NGG 10 8 17 8 24 0 100 5 10
    240606142550_wgiu TTCTCGCTCCCTGGAGAGCCTGGAAGACACTG(+AAAAAA)GATCCTCAGAGAGACAAGGCGGCGTGCTGAC NGG 10 8 17 8 24 0 100 5 10
    240606142548_bum1 AGGCTGTCAGCACGCCGCCTTGTCTCTCTGAG(+AAAAAA)GATCCAGTGTCTTCCAGGCTCTCCAGGGAGC NGG 10 8 17 8 24 0 100 5 10
    240606142547_8afy GTAAATGCTAACAGGGGTGGGAGGCTCTGGTG(+AAAAAA)GATCCTGACAGACACAACCCATAGACACTGC NGG 10 8 17 8 24 0 100 5 10
    240606142545_x3o5 TGGTTGCAGTGTCTATGGGTTGTGTCTGTCAG(+AAAAAA)GATCCACCAGAGCCTCCCACCCCTGTTAGCA NGG 10 8 17 8 24 0 100 5 10
    240606142544_uoek TCTCTGTTAAGTCGATGTGGACTCCAGTCCAG(+AAAAAA)GTACCTGAATGGTTTACCTACGCACAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606142542_rdq5 CACCGGGCTCTGTGCGTAGGTAAACCATTCAG(+AAAAAA)GTACCTGGACTGGAGTCCACATCGACTTAAC NGG 10 8 17 8 24 0 100 5 10
    240606142541_6pae TTAGGATCTAGACCTCTCCTCTGAGCGCTCTG(+AAAAAA)GATCCAAATCTTAGCACCAGCTTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10