Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142645_hpv5 CGCCTGGTAAGACATGAGGAGACTGATGTGTG(+AAAAAA)GTACCCAAACACAGGTAACCAGCCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240606142643_3tr8 GTAACGCCCCAGCCGCAAGGCTGCCTCGCTGG(+AAAAAA)GTACCCATGTGGCAACCAGCGATGTGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606142641_mn3z GCAACCTGACCACATCGCTGGTTGCCACATGG(+AAAAAA)GTACCCAGCGAGGCAGCCTTGCGGCTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606142640_sy6w CTGGCTTCTGAAACAAGCAAACCCCTTTAAAG(+AAAAAA)GTACCCTGGGAAGTCAAGTTTGCTAGCAGTC NGG 10 8 17 8 24 0 100 5 10
    240606142637_w2g3 TCTCAGACTGCTAGCAAACTTGACTTCCCAGG(+AAAAAA)GTACCTTTAAAGGGGTTTGCTTGTTTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240606142636_9mi5 TGAATTACTTCATAAAACTAGATCTTTTTTTG(+AAAAAA)GTACCTGTAACAAAAGCAGATATTTTATTAA NGG 10 8 17 8 24 0 100 5 10
    240606142635_uil1 TTTATTTAATAAAATATCTGCTTTTGTTACAG(+AAAAAA)GTACCAAAAAAAGATCTAGTTTTATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606142633_fk5y AATGCCTTAGGGGCATCATACGTTCTATACGG(+AAAAAA)GTACCAAAAAGCTTACACAGCTTTGTTATAG NGG 10 8 17 8 24 0 100 5 10
    240606142632_kujd TTTATCTATAACAAAGCTGTGTAAGCTTTTTG(+AAAAAA)GTACCCGTATAGAACGTATGATGCCCCTAAG NGG 10 8 17 8 24 0 100 5 10
    240606142630_5fqi AGTTATACCACTGAAGAATATGAAATTCCAGG(+AAAAAA)GATCCAAGAGATGAAACTCCCCTTCCCCAGT NGG 10 8 17 8 24 0 100 5 10