Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142540_n3oi GCGAAGAAGGAAGAAGCTGGTGCTAAGATTTG(+AAAAAA)GATCCAGAGCGCTCAGAGGAGAGGTCTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606142538_vzs8 CTCCCAAAGATGGCAAGGTCTAACCATACATG(+AAAAAA)GATCCTCTGGCTTTGGTTTCTGCGTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606142537_xga0 AGTATTACTTAACGCAGAAACCAAAGCCAGAG(+AAAAAA)GATCCATGTATGGTTAGACCTTGCCATCTTT NGG 10 8 17 8 24 0 100 5 10
    240606142535_9719 ATTTGTTTTATATGAAATAATTTATTTTTCAG(+AAAAAA)GTACCATTTTTTTTTTCATTTTAACTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606142534_ahe4 GTAAAAACAAAGTTAAAATGAAAAAAAAAATG(+AAAAAA)GTACCTGAAAAATAAATTATTTCATATAAAA NGG 10 8 17 8 24 0 100 5 10
    240606142532_v4q2 CCTACTGGGACCCGAGGTAACTCCTGGGGAGG(+AAAAAA)GATCCCTGGGGTTCATCATACTCAAGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606142529_ma7a TTCCTGACCCCTTGAGTATGATGAACCCCAGG(+AAAAAA)GATCCCTCCCCAGGAGTTACCTCGGGTCCCA NGG 10 8 17 8 24 0 100 5 10
    240606142527_xs2k GTCCACTGAGGTGGGGAGAGTGTGCTGGGGGG(+AAAAAA)GATCCAGATGCTAGAGCCACACACAAAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606142526_i5ti CATTACCCCTTTGTGTGTGGCTCTAGCATCTG(+AAAAAA)GATCCCCCCCAGCACACTCTCCCCACCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606142523_vvft TCGGTCGGACATGGAGTAGTTTGCTTTCCTGG(+AAAAAA)GTACCCCTTGCTACAGCACCGTTGTCCTGTG NGG 10 8 17 8 24 0 100 5 10