Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142425_4u09 CACCCTTCTGCAAAATGTCAGCAGGTTCCTAG(+AAAAAA)GTACCATTCTGCATCTATCCCAGGGCATCCT NGG 10 8 17 8 24 0 100 5 10
    240606142423_0hwm GAACCCAGAGCGTGGTGCTCAACAGTCACCTG(+AAAAAA)GTACCAGCTGACTGGATGACTCAGAAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240606142422_icbj CAACCCTAACTTCTGAGTCATCCAGTCAGCTG(+AAAAAA)GTACCAGGTGACTGTTGAGCACCACGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606142420_3vj0 CACACCAAGGGACACCCACAGCACATCTTTGG(+AAAAAA)GTACCCAGAGACAAACCCACAGACTGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606142419_j0s0 CACCAACCTCCAGTCTGTGGGTTTGTCTCTGG(+AAAAAA)GTACCCAAAGATGTGCTGTGGGTGTCCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606142417_0mj1 ATCTAAAATGCGCACGGATGTCCCAGAGTAGG(+AAAAAA)GATCCTGATGGGTATACACAATTTACAGCTT NGG 10 8 17 8 24 0 100 5 10
    240606142416_ns04 CTCTCAAGCTGTAAATTGTGTATACCCATCAG(+AAAAAA)GATCCCTACTCTGGGACATCCGTGCGCATTT NGG 10 8 17 8 24 0 100 5 10
    240606142414_pn83 TCTGAGAGCCAGATGTTAAGACAAGCTGGTGG(+AAAAAA)GATCCCCACTTTGTGAGAGAAGGGAGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606142412_wzho AGGGGCCTTTCTCCCTTCTCTCACAAAGTGGG(+AAAAAA)GATCCCACCAGCTTGTCTTAACATCTGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606142410_b7q1 ACATATATGTCTGTGTCCTACATGCATGCCTG(+AAAAAA)GTACCCACAGAAGCCAGAAGAGGGCATTAGG NGG 10 8 17 8 24 0 100 5 10