Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142408_j9y0 GAGGACCTAATGCCCTCTTCTGGCTTCTGTGG(+AAAAAA)GTACCAGGCATGCATGTAGGACACAGACATA NGG 10 8 17 8 24 0 100 5 10
    240606142406_0nt9 AAGGGCCGACACAGTGCAAGCAGGGGGCCCTG(+AAAAAA)GATCCTACCCAGATCCCATCCCATCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606142404_uis0 CCAGAGCTGGGGATGGGATGGGATCTGGGTAG(+AAAAAA)GATCCAGGGCCCCCTGCTTGCACTGTGTCGG NGG 10 8 17 8 24 0 100 5 10
    240606142402_clnp TCTACAAAACTCCATGGAGCCATGAAATTGGG(+AAAAAA)GTACCTAAATCTATGCTTCTAGGGCCATGGT NGG 10 8 17 8 24 0 100 5 10
    240606142401_otrw CAGTGACCATGGCCCTAGAAGCATAGATTTAG(+AAAAAA)GTACCCCAATTTCATGGCTCCATGGAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606142400_6ufm TGGAGTAGGGAAAAGGCCGACTCAGCCTAACG(+AAAAAA)GATCCCCCTGAGTCCCAGACAAAAGCCACTC NGG 10 8 17 8 24 0 100 5 10
    240606142358_eiqm CAACTGAGTGGCTTTTGTCTGGGACTCAGGGG(+AAAAAA)GATCCGTTAGGCTGAGTCGGCCTTTTCCCTA NGG 10 8 17 8 24 0 100 5 10
    240606142356_p3l5 GCTAATTTAATATTTGTATTTATCCATGCGTG(+AAAAAA)GATCCCTCTGCCACGCAACTGCTGGGTTATT NGG 10 8 17 8 24 0 100 5 10
    240606142355_gzlz TAATCAATAACCCAGCAGTTGCGTGGCAGAGG(+AAAAAA)GATCCACGCATGGATAAATACAAATATTAAA NGG 10 8 17 8 24 0 100 5 10
    240606142353_ftob TTCTGGCAGAAACCCCAAGATATTGCTCAAGG(+AAAAAA)GTACCCAATGCTACTTTACTTTCTATAAAGC NGG 10 8 17 8 24 0 100 5 10