Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142320_8ip4 GAAGTCAGCACATTCCCCATATAAACAGACCG(+AAAAAA)GATCCACAGTATTAACAGACGAAAATTCCCA NGG 10 8 17 8 24 0 100 5 10
    240606142318_jpkr CCCAGAACAGAGGAATAGACAGGCTGTAAAGG(+AAAAAA)GTACCGGAATCAGCTCTGCTGCCTGAGATCT NGG 10 8 17 8 24 0 100 5 10
    240606142316_um63 GCCACAGATCTCAGGCAGCAGAGCTGATTCCG(+AAAAAA)GTACCCTTTACAGCCTGTCTATTCCTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240606142314_9ejv TCACAGAAGACCTAGGACGAGACACTCCTGTG(+AAAAAA)GTACCCTCGGCTGAAGATGCCCCGCCCCACT NGG 10 8 17 8 24 0 100 5 10
    240606142312_06sn ATGAGAGTGGGGCGGGGCATCTTCAGCCGAGG(+AAAAAA)GTACCACAGGAGTGTCTCGTCCTAGGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606142310_wgwq GGAGTACCCGTTGAAGACTGGGCCGATCTAGG(+AAAAAA)GTACCTAGCTTCAAGGTCTGTTGCAGTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606142309_rsmi TGGAGATTTACTGCAACAGACCTTGAAGCTAG(+AAAAAA)GTACCCTAGATCGGCCCAGTCTTCAACGGGT NGG 10 8 17 8 24 0 100 5 10
    240606142307_ii83 ATGTCCCAGACACACAGGACCAGCTAAGGCTG(+AAAAAA)GATCCCTCTTCAGAGAGATTGGGGAGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606142305_fzcq AAGCCTCTCACTCCCCAATCTCTCTGAAGAGG(+AAAAAA)GATCCAGCCTTAGCTGGTCCTGTGTGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606142304_lkzp TGGGCCTGGCCTGGCCAGTGCTTGGTGGAAAG(+AAAAAA)GATCCACAGCTCCCTGGGGCCCCAAGTTGTG NGG 10 8 17 8 24 0 100 5 10