Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142218_f6n9 AGAGATCTGTATTCACTTGGGATGGAGTTTAG(+AAAAAA)GATCCTGCTCGGAGTGGGAACTGTTCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606142216_ppif CCAGGCCGTGCTGGGGTGCTCTCTCTGCTGGG(+AAAAAA)GATCCTTATGGGGCCGGAGGCCACCTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606142215_kcuf ATGCTTTCCTAGGTGGCCTCCGGCCCCATAAG(+AAAAAA)GATCCCCAGCAGAGAGAGCACCCCAGCACGG NGG 10 8 17 8 24 0 100 5 10
    240606142213_4g1r TCTATGAGTATCTGTAAATGTTTTCTGAAGAG(+AAAAAA)GATCCACTGAGCAGCTGTAACCCATCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606142212_0rxr CCACACTGGAGATGGGTTACAGCTGCTCAGTG(+AAAAAA)GATCCTCTTCAGAAAACATTTACAGATACTC NGG 10 8 17 8 24 0 100 5 10
    240606142211_z543 GTTCAAGGTCAGAGAGGTCTGGGTGTGGGCTG(+AAAAAA)GATCCCATGCAGATGTTTGGTAGGTTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606142209_05ck TGGGGTGCTCAACCTACCAAACATCTGCATGG(+AAAAAA)GATCCAGCCCACACCCAGACCTCTCTGACCT NGG 10 8 17 8 24 0 100 5 10
    240606142208_wael GGGGAGGTGAGACAGCTCAAATTTTGATCATG(+AAAAAA)GATCCAACCTCACGGGCACTAGACAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606142206_27du GTGAAACACTCTGTCTAGTGCCCGTGAGGTTG(+AAAAAA)GATCCATGATCAAAATTTGAGCTGTCTCACC NGG 10 8 17 8 24 0 100 5 10
    240606142205_tz68 CAATCCTATCATGCAAGATTGAGGCCCTTAGG(+AAAAAA)GTACCTGACAACGGGCACTAATACACCCCAT NGG 10 8 17 8 24 0 100 5 10