Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142246_6hfg GGGGTAGGTGTAGAACCTGCTCCAGGTATCAG(+AAAAAA)GTACCTTGAATATACCCGTGTTTGTGGTAGG NGG 10 8 17 8 24 0 100 5 10
    240606142245_2upe ACCGCAGTTCCAGAAGCTTCGCCCTTCACAGG(+AAAAAA)GATCCACGCCCTCACTCGCTGTGTCACATGA NGG 10 8 17 8 24 0 100 5 10
    240606142243_yh71 GCTCCTCATGTGACACAGCGAGTGAGGGCGTG(+AAAAAA)GATCCCTGTGAAGGGCGAAGCTTCTGGAACT NGG 10 8 17 8 24 0 100 5 10
    240606142241_zo5a CATGTTTGGAAAGCCCCACCCCTACAGCCCTG(+AAAAAA)GATCCTCTGTTCAGAATCAAAGCAGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606142240_ctaw GCAGGGAAGGGCTGCTTTGATTCTGAACAGAG(+AAAAAA)GATCCAGGGCTGTAGGGGTGGGGCTTTCCAA NGG 10 8 17 8 24 0 100 5 10
    240606142238_24mp AACACAAGGCTGCCATCCTGCAGCTCCAGAGG(+AAAAAA)GATCCCAGCCCTGGACCAAGGTCTGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240606142237_b33x TACTTAGTTGCCAGACCTTGGTCCAGGGCTGG(+AAAAAA)GATCCCTCTGGAGCTGCAGGATGGCAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606142235_lwul TTTTCAATTATAAGCAAGACAGGTGCAGACAG(+AAAAAA)GTACCATGAGACCCTCAGAGGCAGGAGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606142234_lzc0 CCGCCAGCACTCCTGCCTCTGAGGGTCTCATG(+AAAAAA)GTACCTGTCTGCACCTGTCTTGCTTATAATT NGG 10 8 17 8 24 0 100 5 10
    240606142233_13r3 GGTTCCCTTCCAGTATGCAGTTAATCATGATG(+AAAAAA)GATCCTGTATATACGAGCTCCTCCGTCCCCT NGG 10 8 17 8 24 0 100 5 10