Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141811_hbwf GTGCACCACCTTTCTGTGAATCTCTCCCACTG(+AAAAAA)GATCCCTCTTACCTGCAACGTGTCTGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240606141810_8faz TACTTGAGCACCACACAAAGGCTGGGATAAGG(+AAAAAA)GTACCTTCTGATGTTTTCTCAGAGCTGGGCG NGG 10 8 17 8 24 0 100 5 10
    240606141809_zb92 TTTCCCGCCCAGCTCTGAGAAAACATCAGAAG(+AAAAAA)GTACCCTTATCCCAGCCTTTGTGTGGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606141807_onde GCCCCAGTCACTTCCCACTCCCTGCTCTCCAG(+AAAAAA)GTACCACCCGGGTCAGTGAGATACTTGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606141805_oj9t AGACCATGCCAAGTATCTCACTGACCCGGGTG(+AAAAAA)GTACCTGGAGAGCAGGGAGTGGGAAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240606141803_nnz8 TCTCACAACCACCTACAACTCCAATTCCAAGG(+AAAAAA)GATCCAATGGCATCTTCTGGCTTCTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606141801_wk92 CCTGGTCCCTCAGAAGCCAGAAGATGCCATTG(+AAAAAA)GATCCCTTGGAATTGGAGTTGTAGGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606141800_9xoo TAGTCCCAGGATTGGGGAGGTAGAGACAGGAG(+AAAAAA)GATCCAGAGTTCATGGTCCTCAGCTACATAG NGG 10 8 17 8 24 0 100 5 10
    240606141758_7y5c GTTTCCTATGTAGCTGAGGACCATGAACTCTG(+AAAAAA)GATCCTCCTGTCTCTACCTCCCCAATCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606141757_1dwm TACTGGCCTGAGGTGACAGGGTGGGAGAGCTG(+AAAAAA)GATCCATGAGGGAGCATGGTAGCCCGAAAGC NGG 10 8 17 8 24 0 100 5 10