Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141726_uy8a TTCCTAGAGCTTTCCAGCTCCATGGTTACGAG(+AAAAAA)GTACCGTCTCTCTGGTCTTTGATTTCCAACA NGG 10 8 17 8 24 0 100 5 10
    240606141725_n4jc GGAAGAGAGCCTGGCAAAGAGGTGAAAATGAG(+AAAAAA)GTACCAAAAGAGAAACTGGCATCTTTGGGAT NGG 10 8 17 8 24 0 100 5 10
    240606141724_1t5u TTTTAATCCCAAAGATGCCAGTTTCTCTTTTG(+AAAAAA)GTACCTCATTTTCACCTCTTTGCCAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606141722_o3ge AGCAAACCAGCAGTGGAACGCCTCAGAACAGG(+AAAAAA)GATCCAAAGGGTACACAGGAATCAAGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606141720_s1v1 GTATCTCTCACTTGATTCCTGTGTACCCTTTG(+AAAAAA)GATCCCTGTTCTGAGGCGTTCCACTGCTGGT NGG 10 8 17 8 24 0 100 5 10
    240606141719_1ryw CCAGGACAAATCAGTTCCAGCCTGGATTCATG(+AAAAAA)GATCCCAGTCTCAGGCCACATATGGATTTGG NGG 10 8 17 8 24 0 100 5 10
    240606141717_4ach TGGTCCCAAATCCATATGTGGCCTGAGACTGG(+AAAAAA)GATCCATGAATCCAGGCTGGAACTGATTTGT NGG 10 8 17 8 24 0 100 5 10
    240606141716_gcgz ATTCTCTTCAGACACACCAGAAGACAGCATTG(+AAAAAA)GATCCCAATTACAGATGGTTATGACCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606141714_e7si ACCACATGGTGGTCATAACCATCTGTAATTGG(+AAAAAA)GATCCAATGCTGTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606141712_2ncr GTCAATGACTGAGGTCAGCAACCAACTGCTGG(+AAAAAA)GATCCAAGGATGGTGCTAATTTCTTCTGTCA NGG 10 8 17 8 24 0 100 5 10