Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141642_pnv4 ACCAAAGCATCTGCCTCTTTTGGAGGCTGAGG(+AAAAAA)GATCCATAGGTTCAACCCACGGCTTTACATA NGG 10 8 17 8 24 0 100 5 10
    240606141640_p8lm AGTGTCTGGGTTTGATGGCTGATTATGGGATG(+AAAAAA)GATCCCCGGGTGGGGTAGTCTCTGGAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606141637_m9vk GGATGGACTTTCCAGAGACTACCCCACCCGGG(+AAAAAA)GATCCATCCCATAATCAGCCATCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606141636_qtcq AAACTGTCCAAAGCACTGGACTGACATCACTG(+AAAAAA)GTACCAGGGACGCCTACCTTTCTGGGGTATT NGG 10 8 17 8 24 0 100 5 10
    240606141633_1y8w TTACAAATACCCCAGAAAGGTAGGCGTCCCTG(+AAAAAA)GTACCAGTGATGTCAGTCCAGTGCTTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240606141631_ipoo GTTCTTATAAAGCCACTAATCCTATCATGAGG(+AAAAAA)GATCCACCTTCATGAACTCCACTTATCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606141630_t7va CTTATCCAGGATAAGTGGAGTTCATGAAGGTG(+AAAAAA)GATCCCTCATGATAGGATTAGTGGCTTTATA NGG 10 8 17 8 24 0 100 5 10
    240606141629_tgyy GAACTTTAGCTTCTCCGAAGCCAGGGAGCAAG(+AAAAAA)GATCCCAGTTCCAGGTCCTGACATCAGTTTC NGG 10 8 17 8 24 0 100 5 10
    240606141627_488d ACTCAGAAACTGATGTCAGGACCTGGAACTGG(+AAAAAA)GATCCTTGCTCCCTGGCTTCGGAGAAGCTAA NGG 10 8 17 8 24 0 100 5 10
    240606141626_4xgq TAAAGACCACAGTAGAGAAGTAGTTAGTGCTG(+AAAAAA)GTACCATCCACAGCCACTAGGAACTAACCAC NGG 10 8 17 8 24 0 100 5 10