Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141541_lpht TCGTACTCATTAACGTTTGTGTCTAGGTACTG(+AAAAAA)GTACCTTTCTGTTTAAAAAAATGTTCTAAGT NGG 10 8 17 8 24 0 100 5 10
    240606141540_7e83 CCAGAGCATATACAAAATTATACAAATTACAG(+AAAAAA)GTACCTGGTTTGTAAAACTGACCATCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606141538_v8ty GAAGTCACCTGATGGTCAGTTTTACAAACCAG(+AAAAAA)GTACCTGTAATTTGTATAATTTTGTATATGC NGG 10 8 17 8 24 0 100 5 10
    240606141536_g1gg ATCTACTTGTGGTTCCCCAGACAGCTCTAGGG(+AAAAAA)GATCCGAACTGACTCTGTCCATGCATTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606141535_filq ACCCCTGAGAATGCATGGACAGAGTCAGTTCG(+AAAAAA)GATCCCCTAGAGCTGTCTGGGGAACCACAAG NGG 10 8 17 8 24 0 100 5 10
    240606141533_ujkc CTTCGGGGGAAAGACCCACCCTAACTGTGGTG(+AAAAAA)GTACCGTTTCATAGGCTGGGGTCCCAGACTA NGG 10 8 17 8 24 0 100 5 10
    240606141532_sehj TTATCTAGTCTGGGACCCCAGCCTATGAAACG(+AAAAAA)GTACCACCACAGTTAGGGTGGGTCTTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606141531_f2im GTTAGGAGAGGTTAGGAAACCTCTAGAAAGTG(+AAAAAA)GATCCTTGCTGGGGGTAATTATATCACTGGG NGG 10 8 17 8 24 0 100 5 10
    240606141530_go8t CCTACCCCAGTGATATAATTACCCCCAGCAAG(+AAAAAA)GATCCACTTTCTAGAGGTTTCCTAACCTCTC NGG 10 8 17 8 24 0 100 5 10
    240606141528_s0k1 GTTTTCACTAGGTATAGAGCAGTAGTCACCAG(+AAAAAA)GTACCAACACAGGGAGCCCTCCACACACTCT NGG 10 8 17 8 24 0 100 5 10