Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141527_avwa TCTTCAGAGTGTGTGGAGGGCTCCCTGTGTTG(+AAAAAA)GTACCTGGTGACTACTGCTCTATACCTAGTG NGG 10 8 17 8 24 0 100 5 10
    240606141526_kbys TCAAGAACTCTGTCTTACCATTTCTTGTCTAG(+AAAAAA)GTACCTAGCATGGCAAACCTGTGGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606141524_cwvp AATGACTCTCTCCCACAGGTTTGCCATGCTAG(+AAAAAA)GTACCTAGACAAGAAATGGTAAGACAGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606141523_q4bw GATTTTGGCCTTTTTCAGTTGGGTACTATATG(+AAAAAA)GTACCAGGATGAATACATGGCCTTTAATGTT NGG 10 8 17 8 24 0 100 5 10
    240606141521_0z14 GAAGGAACATTAAAGGCCATGTATTCATCCTG(+AAAAAA)GTACCATATAGTACCCAACTGAAAAAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606141520_wr02 GCTTGCTTCATAAGCATGAAGACTGCCATTTG(+AAAAAA)GATCCTAGTACCCAAACAAAAGCTGGGCATT NGG 10 8 17 8 24 0 100 5 10
    240606141519_whir GGGACAATGCCCAGCTTTTGTTTGGGTACTAG(+AAAAAA)GATCCAAATGGCAGTCTTCATGCTTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240606141517_4dqa GGACAGCTCACCATTATGATCTGTACCAGTTG(+AAAAAA)GTACCTGCCTTGGTCAGCAGGGAGCTGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606141515_hbfa TATGATCCTCAGCTCCCTGCTGACCAAGGCAG(+AAAAAA)GTACCAACTGGTACAGATCATAATGGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606141514_yiae GTTCACTAAGTAACTTGTGCTCATTTTCATTG(+AAAAAA)GATCCTGCAGTGACTCATTTCCATATGGTCG NGG 10 8 17 8 24 0 100 5 10