Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141431_01f2 TGTGTATATATGTGTATATATATACACACATG(+AAAAAA)GTACCTTATTCATTTGGGGTCTTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240606141429_n85m TTTAGTCACAATAAGGACCCCAAATGAATAAG(+AAAAAA)GTACCATGTGTGTGTGTGTGTGTGTGTATAC NGG 10 8 17 8 24 0 100 5 10
    240606141428_yk2d TATGTGTATACACACACACACACACACACATG(+AAAAAA)GTACCTTATTCATTTGGGGTCCTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240606141427_bqqt TAGTGTTTAAAGCATGTGATGCAGTGGCAGAG(+AAAAAA)GATCCAGGTTTAGTTCCTAGCACCTGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606141425_ykbr AGGTGCCACACAGGTGCTAGGAACTAAACCTG(+AAAAAA)GATCCTCTGCCACTGCATCACATGCTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606141424_2n6v GTGGTAAGTGATTAACCATGTTCTGGTGGCGG(+AAAAAA)GTACCAAACTGTTCTTCATGATAGAGTAGCT NGG 10 8 17 8 24 0 100 5 10
    240606141422_mzov GAAGCAGCTACTCTATCATGAAGAACAGTTTG(+AAAAAA)GTACCCGCCACCAGAACATGGTTAATCACTT NGG 10 8 17 8 24 0 100 5 10
    240606141421_j2h0 TCATAGTGGTCCACTGAGGTCATATATTTCTG(+AAAAAA)GATCCTTCCGTCTTAGGAAATATCAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240606141419_io0w CAGAAGAGGGTTGATATTTCCTAAGACGGAAG(+AAAAAA)GATCCAGAAATATATGACCTCAGTGGACCAC NGG 10 8 17 8 24 0 100 5 10
    240606141418_5wfd CAGAGTTCCCTGTAGATGGACTGATTCACCAG(+AAAAAA)GATCCTGCTACACTGAAGGCGCACAGTTGAC NGG 10 8 17 8 24 0 100 5 10