Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141513_pqng CAATGCGACCATATGGAAATGAGTCACTGCAG(+AAAAAA)GATCCAATGAAAATGAGCACAAGTTACTTAG NGG 10 8 17 8 24 0 100 5 10
    240606141511_diwt GCTACATCTCCCAGACTTTCAGTCTTATCTAG(+AAAAAA)GATCCTCTCCTCTTCCCAGCCTGAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606141510_1e0x TGGGGCCTGGGTTCAGGCTGGGAAGAGGAGAG(+AAAAAA)GATCCTAGATAAGACTGAAAGTCTGGGAGAT NGG 10 8 17 8 24 0 100 5 10
    240606141508_8ncj TAATTGTATTTGGTTGTTTGTGCTTGCAGGTG(+AAAAAA)GTACCACCTAGGCAAATGTCTGACCCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606141507_pcv0 CTATGAGGAGGGGTCAGACATTTGCCTAGGTG(+AAAAAA)GTACCACCTGCAAGCACAAACAACCAAATAC NGG 10 8 17 8 24 0 100 5 10
    240606141505_xq2o CAAAAAACCAGGCCATTCCTTCACTTCAAAGG(+AAAAAA)GTACCACTTTCCTTATGGATGGACCAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240606141504_wm6q ACGTGATTCCTGGTCCATCCATAAGGAAAGTG(+AAAAAA)GTACCCTTTGAAGTGAAGGAATGGCCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606141502_io1h TCAATCTTGTTTTACAGCTTATTTCAGAGAGG(+AAAAAA)GATCCTGGAAATATTTACAGAGTTTTTTTAG NGG 10 8 17 8 24 0 100 5 10
    240606141500_5yum TAAGGCTAAAAAAACTCTGTAAATATTTCCAG(+AAAAAA)GATCCCTCTCTGAAATAAGCTGTAAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240606141459_gcaq GAGCCTCTGTACAGGAATCTTCAAATAACATG(+AAAAAA)GTACCCCTAAAACTGAGAATTGTTTCTAGGC NGG 10 8 17 8 24 0 100 5 10