Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141457_4igs ACCTTGCCTAGAAACAATTCTCAGTTTTAGGG(+AAAAAA)GTACCATGTTATTTGAAGATTCCTGTACAGA NGG 10 8 17 8 24 0 100 5 10
    240606141456_lxle TCGAGTTCCAGATTTTGATCTACACAAACATG(+AAAAAA)GATCCTCTGTGTAGTCTTGGCTGTCCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606141455_smfd GTGAGTTCCAGGACAGCCAAGACTACACAGAG(+AAAAAA)GATCCATGTTTGTGTAGATCAAAATCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606141454_yuy2 CTGTATCTACCCAGGCTATGAGCCAGGATCTG(+AAAAAA)GTACCACCCTCCTTCTGTACCAAGAACACTG NGG 10 8 17 8 24 0 100 5 10
    240606141452_wx9a TACCACAGTGTTCTTGGTACAGAAGGAGGGTG(+AAAAAA)GTACCAGATCCTGGCTCATAGCCTGGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606141451_7y0b CCCAGCAGGGGCAGGAGAGGACTGGTCTCATG(+AAAAAA)GATCCCCTCTCCCCTGTTCTGCGGGCACCTC NGG 10 8 17 8 24 0 100 5 10
    240606141449_t4nz CAGCGGAGGTGCCCGCAGAACAGGGGAGAGGG(+AAAAAA)GATCCATGAGACCAGTCCTCTCCTGCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606141447_0q4k TTCTGTGTGTAGGGGTCTGTCCATGATGTTGG(+AAAAAA)GTACCACCTGAATTAATCCCGGATTTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240606141446_ucza ACACATAACAAAATCCGGGATTAATTCAGGTG(+AAAAAA)GTACCCAACATCATGGACAGACCCCTACACA NGG 10 8 17 8 24 0 100 5 10
    240606141444_7wuz ACACACAGCATGTAATTAACAACAGATCTATG(+AAAAAA)GATCCAGCTCTTAATATTGATCCTTTTGGTG NGG 10 8 17 8 24 0 100 5 10