Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141401_yt9p CAGAAAGGGGACTTTTGGCCATGCTGACCATG(+AAAAAA)GTACCTTAGATGCTGAGGGCTGAGGCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606141400_p8bk AGACCCTAGGGAGAAGAGACAAGTCCTGTAGG(+AAAAAA)GATCCTATTGCCAGTGCTAGCTCAAGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606141358_2gm8 CGAGAACAGGCTTGAGCTAGCACTGGCAATAG(+AAAAAA)GATCCCTACAGGACTTGTCTCTTCTCCCTAG NGG 10 8 17 8 24 0 100 5 10
    240606141356_yxxf CTCCTATGCAGACAGCTGCCATTCCTGCCAAG(+AAAAAA)GTACCTAGGCTGAGGTTCAGCCTCCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240606141355_rd25 CCTTGGATCTGGGAGGCTGAACCTCAGCCTAG(+AAAAAA)GTACCTTGGCAGGAATGGCAGCTGTCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240606141353_2zti AAGGCCACCAGCGAGGCCGAGCAAGGCCTCTG(+AAAAAA)GTACCCACTGACTACCCTAGAGCTCCCCTTT NGG 10 8 17 8 24 0 100 5 10
    240606141351_msf8 CTCCCAAAGGGGAGCTCTAGGGTAGTCAGTGG(+AAAAAA)GTACCAGAGGCCTTGCTCGGCCTCGCTGGTG NGG 10 8 17 8 24 0 100 5 10
    240606141349_3ht0 CCAATTAAATTAAAACCTTAGCTAGGACCCAG(+AAAAAA)GTACCAGTATGTTTATTGGGAGCCACTGATA NGG 10 8 17 8 24 0 100 5 10
    240606141348_monx CTATGTATCAGTGGCTCCCAATAAACATACTG(+AAAAAA)GTACCTGGGTCCTAGCTAAGGTTTTAATTTA NGG 10 8 17 8 24 0 100 5 10
    240606141346_0r8w CTGGAGACCGTGGGGTCTAGGTAGCCTGCCAG(+AAAAAA)GATCCCAGCAGTGAGCTCACTTTGGGCCATT NGG 10 8 17 8 24 0 100 5 10