Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141311_y3ld GGTGCCTGAGGCCATTATTTCCACTGCTAAGG(+AAAAAA)GATCCCGTTCCCAGAAGGTGGCATCCATCCA NGG 10 8 17 8 24 0 100 5 10
    240606141309_hf03 TTGAAATGTAAAGCCTGTCCATATGTTTTAAG(+AAAAAA)GATCCATGTATTTCTGTTTTGTTTGTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606141308_pjnq GTTTTGAAAAACAAACAAAACAGAAATACATG(+AAAAAA)GATCCTTAAAACATATGGACAGGCTTTACAT NGG 10 8 17 8 24 0 100 5 10
    240606141307_4yuc CTTTGGGAACTGAGGGGACTCAGGGTCATTGG(+AAAAAA)GTACCAGTGGGGCCAGTTCGTGAGATTAGCT NGG 10 8 17 8 24 0 100 5 10
    240606141305_qrot TGGAAAGCTAATCTCACGAACTGGCCCCACTG(+AAAAAA)GTACCCAATGACCCTGAGTCCCCTCAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606141303_pwr1 GCTCTCTTCAGACACACCAGAAGAGGGCATCG(+AAAAAA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606141302_dgax ACCACATGGTGGCTCACAACCATCTGTAATGG(+AAAAAA)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606141300_53aw GAATCCCTTTTCCTACATGTCCTGGGAAGCTG(+AAAAAA)GATCCCTGGCCTTCCCACCTTCCCACCCCCT NGG 10 8 17 8 24 0 100 5 10
    240606141258_t1mw GGGTGAGGGGGTGGGAAGGTGGGAAGGCCAGG(+AAAAAA)GATCCAGCTTCCCAGGACATGTAGGAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606141257_jltt TTAAGTGGATTGACAATGACAGAACATGTCTG(+AAAAAA)GATCCATCCTTAGACATGCTTTTGCTGAAGT NGG 10 8 17 8 24 0 100 5 10