Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141242_7i8e CACTTGACAGATGATCTAACCCTGAAGGCCTG(+AAAAAA)GTACCATCCACCCTAGAGTCCTTTCTTCCAA NGG 10 8 17 8 24 0 100 5 10
    240606141240_ursg CTAGAGAGCACCAGCTCCAGGTCCATGACTGG(+AAAAAA)GTACCACTGTTTGCCCTGGCCTGTCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606141239_4kz8 GCTCCTCAGGAGACAGGCCAGGGCAAACAGTG(+AAAAAA)GTACCCAGTCATGGACCTGGAGCTGGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606141237_vgxf TGTCACCAGGGGGTGTGCAAGCCGGCATAAGG(+AAAAAA)GATCCCTTGGAGATACTCAAGAAGAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606141235_plrl CAGGGAGCCCCTCTTCTTGAGTATCTCCAAGG(+AAAAAA)GATCCCTTATGCCGGCTTGCACACCCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606141233_apai AGTCTTCAGCAACCACCATGGCCTGATATTGG(+AAAAAA)GTACCACCCTGCAGGTTATCAGCTACACTCC NGG 10 8 17 8 24 0 100 5 10
    240606141232_2od4 ATATGGGAGTGTAGCTGATAACCTGCAGGGTG(+AAAAAA)GTACCCAATATCAGGCCATGGTGGTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606141229_d32l ACTGCTCTGAGCTTAGCACACTCCTGTCCCAG(+AAAAAA)GATCCTTCCTGCCTCCCATCCCCCATATCAC NGG 10 8 17 8 24 0 100 5 10
    240606141227_e7wk AGCAGGTGATATGGGGGATGGGAGGCAGGAAG(+AAAAAA)GATCCTGGGACAGGAGTGTGCTAAGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606141225_jvq5 AAGCAGTGGAAAGAGAACAGGTTGTTTCCCTG(+AAAAAA)GTACCTTAGTACTGTGGAACAAAAATAACCC NGG 10 8 17 8 24 0 100 5 10