Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141154_7yav AAAATTTATGCTTTAAGTCCCTCACAAGGGGG(+AAAAAA)GTACCCGCTAGCAACCTATCAAAAGTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606141153_7vk0 TTCTGGTTTCTGGAACTAGTGAGAGGCATGAG(+AAAAAA)GATCCAAGAAAAAGGCAGAGAGCAAGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606141152_dhsa AGTTAACCTACTTGCTCTCTGCCTTTTTCTTG(+AAAAAA)GATCCTCATGCCTCTCACTAGTTCCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240606141151_4hal AGATAGAAAAATTGTCAGTACACTAGCTCTAG(+AAAAAA)GATCCAGAATGCAGGTGGCTGGTTTCACCCA NGG 10 8 17 8 24 0 100 5 10
    240606141149_82kc CCCTTTGGGTGAAACCAGCCACCTGCATTCTG(+AAAAAA)GATCCTAGAGCTAGTGTACTGACAATTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606141148_7xq9 GGTTTCATACCATTTGAGCCAGTGTCATGGTG(+AAAAAA)GTACCCATACTCTTTTGTTCAGCTCTCAGTC NGG 10 8 17 8 24 0 100 5 10
    240606141147_mv86 GACAGGACTGAGAGCTGAACAAAAGAGTATGG(+AAAAAA)GTACCACCATGACACTGGCTCAAATGGTATG NGG 10 8 17 8 24 0 100 5 10
    240606141145_ksii AACAAAAGAGCTTTGGGCCAGTTTGACAGTTG(+AAAAAA)GTACCCCCTCTAATGCCTCACCTAAATGGGG NGG 10 8 17 8 24 0 100 5 10
    240606141143_1gsl AAACGCCCCATTTAGGTGAGGCATTAGAGGGG(+AAAAAA)GTACCAACTGTCAAACTGGCCCAAAGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606141142_bete AGATTGTTATCAAGGAAACTCAGTAAGAATAG(+AAAAAA)GTACCCAAATAAATCGGGAAGTTTGTATCCT NGG 10 8 17 8 24 0 100 5 10