Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141056_fd4i TTCACTGTTACTTCTGTCTCTTTAGATTGCAG(+AAAAAA)GATCCTTTATCGTCTCACTTTGATCATAGTA NGG 10 8 17 8 24 0 100 5 10
    240606141054_wpb8 TGGGAGTGTTTCCTGCCATGCATGAAGCCTTG(+AAAAAA)GATCCACCCCAGCGTTGTGTGAACTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606141052_k04v CTCCCACACCCAGTTCACACAACGCTGGGGTG(+AAAAAA)GATCCAAGGCTTCATGCATGGCAGGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240606141050_a1th AGACCTGCCTGCCTCTGCCTCCCAAGTGCTGG(+AAAAAA)GATCCAAGGTGTGAGCCACAGCCTGGCTTCA NGG 10 8 17 8 24 0 100 5 10
    240606141048_4pns AGGCCTGAAGCCAGGCTGTGGCTCACACCTTG(+AAAAAA)GATCCCAGCACTTGGGAGGCAGAGGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606141047_zfpv ACGAGAAATGTGGGTTCATTTAGTTTAATAGG(+AAAAAA)GATCCATGATTGAGGACTAGGGTGAGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240606141045_ept5 CCTTTAACTACTCACCCTAGTCCTCAATCATG(+AAAAAA)GATCCCTATTAAACTAAATGAACCCACATTT NGG 10 8 17 8 24 0 100 5 10
    240606141044_ldsw CCCCATGTTAGTTGTAAAATATCAGCTGCTAG(+AAAAAA)GTACCCAGGTTTGCTTTGCAGGGCATATTTT NGG 10 8 17 8 24 0 100 5 10
    240606141042_ngua TCTATAAAATATGCCCTGCAAAGCAAACCTGG(+AAAAAA)GTACCTAGCAGCTGATATTTTACAACTAACA NGG 10 8 17 8 24 0 100 5 10
    240606141040_i2bs TTATTTCCCACGGTCTGCACAGCAGCTGGAGG(+AAAAAA)GTACCACAGCCCGACTGATGGCTACAAAGCC NGG 10 8 17 8 24 0 100 5 10