Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141111_g82d TAGATTATTAACCTAATCTTGATTTAACTTTG(+AAAAAA)GTACCTGGTATCTGTCTAGAAAGTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606141109_wm6t TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AAAAAA)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606141107_p0p6 TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+AAAAAA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606141105_7s7b GGATGGACCATCTAGTGATTGCCATATCCAGG(+AAAAAA)GATCCATCCCATAATCAGCTTCCAAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606141103_al2n GTGTCAGCCTTTTGGAAGCTGATTATGGGATG(+AAAAAA)GATCCCTGGATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606141102_5wu4 AACTCCTGGCCCTTCCATGAACATTCAAAAAG(+AAAAAA)GATCCACTTGTGTTCTAGAACGAGCGCTGTA NGG 10 8 17 8 24 0 100 5 10
    240606141101_v34y GTTCCTACAGCGCTCGTTCTAGAACACAAGTG(+AAAAAA)GATCCTTTTTGAATGTTCATGGAAGGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606141100_7v3m CTTATAGCTGTTGAGTATTTTCAATATCACTG(+AAAAAA)GATCCCTCAGTCTTCACCCATTGGTAAATAT NGG 10 8 17 8 24 0 100 5 10
    240606141058_iy19 CTTATATATTTACCAATGGGTGAAGACTGAGG(+AAAAAA)GATCCAGTGATATTGAAAATACTCAACAGCT NGG 10 8 17 8 24 0 100 5 10
    240606141057_jihm CAGTATACTATGATCAAAGTGAGACGATAAAG(+AAAAAA)GATCCTGCAATCTAAAGAGACAGAAGTAACA NGG 10 8 17 8 24 0 100 5 10