Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141039_w5pr CCAGAGGCTTTGTAGCCATCAGTCGGGCTGTG(+AAAAAA)GTACCCTCCAGCTGCTGTGCAGACCGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606141037_ujhy TTTTTCTTACCTGTAAAATGAAGGTGTAGTAG(+AAAAAA)GTACCACCTAGTGCTGTGGGGAGCATCACAA NGG 10 8 17 8 24 0 100 5 10
    240606141036_oby4 TTTTCTTGTGATGCTCCCCACAGCACTAGGTG(+AAAAAA)GTACCTACTACACCTTCATTTTACAGGTAAG NGG 10 8 17 8 24 0 100 5 10
    240606141033_iy2n GACATTTTCTACCACTTAATGTAACATGCCTG(+AAAAAA)GTACCCGTCATGCAAAGCTGCAACACACAGC NGG 10 8 17 8 24 0 100 5 10
    240606141031_jf5i AGTGGGCTGTGTGTTGCAGCTTTGCATGACGG(+AAAAAA)GTACCAGGCATGTTACATTAAGTGGTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606141029_0d08 ACGCCCACCATTTATCTGAGTTTTTAAGGTAG(+AAAAAA)GTACCTTATTCCAGCACTCAGAAAGAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606141028_thwy TTCTCCTGCCTCTTTCTGAGTGCTGGAATAAG(+AAAAAA)GTACCTACCTTAAAAACTCAGATAAATGGTG NGG 10 8 17 8 24 0 100 5 10
    240606141026_2u2g CACCCACACCTGTCTGTACCTCACTCCAGAGG(+AAAAAA)GATCCACCGCCCTCTTCTGCCCTCTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606141025_886x CAGGTGCCTGCAGAGGGCAGAAGAGGGCGGTG(+AAAAAA)GATCCCTCTGGAGTGAGGTACAGACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606141022_n9aq CAGCTTTCCTCTCTTTACCTTTCACGCCTTGG(+AAAAAA)GATCCTAAGGATAAAGCCCTGGTTGGTAGTA NGG 10 8 17 8 24 0 100 5 10