Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140947_gu59 CCTCAACACAGTGACAGGCGTTTAATCCCTAG(+AAAAAA)GATCCACTCAGTTCAAGGAGAACCAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240606140946_ehci GACGGGCACGGTGAGCTAGGACAAGGAACGAG(+AAAAAA)GATCCAGGAGTCTGTGTAGGAATTAGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240606140944_7gmh CACCCATCTTCTAATTCCTACACAGACTCCTG(+AAAAAA)GATCCTCGTTCCTTGTCCTAGCTCACCGTGC NGG 10 8 17 8 24 0 100 5 10
    240606140943_vk4m GAGAAGAAGGAGGAGGTACAGGGTGCTTTTTG(+AAAAAA)GTACCAGGGAGAAAGTCATTGTGGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606140941_rqgy AACTACCTCTTCCCACAATGACTTTCTCCCTG(+AAAAAA)GTACCAAAAAGCACCCTGTACCTCCTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606140939_6mwh CCTGGGTTTGTTTGGTGAGGACCCTGTGGTTG(+AAAAAA)GTACCTTAGCTTGGAACAGACCTGGATGAAC NGG 10 8 17 8 24 0 100 5 10
    240606140938_pu1i ACACAGTTCATCCAGGTCTGTTCCAAGCTAAG(+AAAAAA)GTACCAACCACAGGGTCCTCACCAAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240606140936_gvf2 GGCTTTCCTCAGCATGGCCACATCAGCCATGG(+AAAAAA)GTACCTGGAAATGAAGCTCCAGAGCAGGTCC NGG 10 8 17 8 24 0 100 5 10
    240606140934_h7uu GGCAGGGACCTGCTCTGGAGCTTCATTTCCAG(+AAAAAA)GTACCCATGGCTGATGTGGCCATGCTGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606140932_c2l1 CACACCAAGAAGTCCATCCTTACAGTCCCACG(+AAAAAA)GTACCTCTGCGTTCTAGGACTGTAGGACTGT NGG 10 8 17 8 24 0 100 5 10