Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142050_6mv1 ATGGAAAGGATGTAATGTACTTAGGTGTGGCG(+AAAAAA)GATCCTGGGCTAGAGTTGTGGAGGAAAGACG NGG 10 8 17 8 24 0 100 5 10
    240606142048_caq6 GCCACTGAACCGACAGAGTTGCCCTAAGCCGG(+AAAAAA)GTACCTGACCTGAGTTCAGGGTAGCAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606142047_95p1 CTGTCTAGGCTGCTACCCTGAACTCAGGTCAG(+AAAAAA)GTACCCGGCTTAGGGCAACTCTGTCGGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606142044_52es TTAGTGTTCACAGCAGTTAGAACTCACCCCTG(+AAAAAA)GATCCTGCAACCCATTTCCACTGATGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606142042_2zbb ATTTTAACCACATCAGTGGAAATGGGTTGCAG(+AAAAAA)GATCCAGGGGTGAGTTCTAACTGCTGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240606142040_y5f5 GTCACCCCAAACCAAAGCGAAACACAGACCTG(+AAAAAA)GTACCGAAGACACTTCGTATTCCCAAAGCCG NGG 10 8 17 8 24 0 100 5 10
    240606142039_pgxh GCACACGGCTTTGGGAATACGAAGTGTCTTCG(+AAAAAA)GTACCAGGTCTGTGTTTCGCTTTGGTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606142038_bu0e GTTTGTCTGGGTGTAGGGTTGGACAGCTCTGG(+AAAAAA)GATCCGATTAAGGCCATGTTCTGGGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606142036_8v95 TGAGGGGCAGCCCCAGAACATGGCCTTAATCG(+AAAAAA)GATCCCAGAGCTGTCCAACCCTACACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606142035_ghln ATGCTGCCTGTGAAGCTGTGTTCTCAGGTCAG(+AAAAAA)GATCCTGTTCACCAGCGTCAGAAAGTGTACA NGG 10 8 17 8 24 0 100 5 10