Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140737_tjlo AGAATTTGCCTTAATATGATCTTTTTATTTGG(+AAAAAA)GATCCCCTGATTCAATTCGATTTCAGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606140735_85b4 TGGATCCCACAAGGGACCACCAAGTGACCTGG(+AAAAAA)GTACCTCAGTCATGTTAAAACATGTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606140733_jm1e AGGAAAGGAAAACATGTTTTAACATGACTGAG(+AAAAAA)GTACCCAGGTCACTTGGTGGTCCCTTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606140732_s03l TTCATTTATGATTGGCTAACAAGTAACACCTG(+AAAAAA)GATCCCACAAGGGACCACCAAGTGACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606140730_9sj7 AGGTACCCAGGTCACTTGGTGGTCCCTTGTGG(+AAAAAA)GATCCAGGTGTTACTTGTTAGCCAATCATAA NGG 10 8 17 8 24 0 100 5 10
    240606140728_1t4x TGTCTTTTGGCTAATCCTAAGTAGCTCCTTTG(+AAAAAA)GATCCTCCTTCACAGGCTCTGTAGCTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606140726_54xa AGCCATCTACAGCTACAGAGCCTGTGAAGGAG(+AAAAAA)GATCCAAAGGAGCTACTTAGGATTAGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240606140725_vpx5 TCGCAAAACTTGGTCTCTGCTGGAGAACACTG(+AAAAAA)GATCCCCTGTGGGTGGGTGGGTGGGCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606140723_umw4 ATTTTCAACAGCCCACCCACCCACCCACAGGG(+AAAAAA)GATCCAGTGTTCTCCAGCAGAGACCAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606140722_fiix AGAGAGCCCTTTACATCCACTCTGTTGGGCAG(+AAAAAA)GATCCAGTCAACCTGCTCAGCCTGCTTAGGC NGG 10 8 17 8 24 0 100 5 10