Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141856_lo78 GTAGCAAACTGAAGTCTACCTCCTGCTGCTTG(+AAAAAA)GTACCAATCACCCCATTTCCTCTTAAACCTT NGG 10 8 17 8 24 0 100 5 10
    240606141854_5yci ATGACAGGCCCAAAACCTCCACAGGAGCCAGG(+AAAAAA)GTACCAGGAGACATGAGAGAACTCCTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606141852_q5v6 ATGGGACAGAAGGAGTTCTCTCATGTCTCCTG(+AAAAAA)GTACCCTGGCTCCTGTGGAGGTTTTGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606141851_whw7 ACAGCTTTGCATGTGTTGCCCTGACCTCAGAG(+AAAAAA)GATCCTTCTTCTGTAGCCTCCAGGGGACTGA NGG 10 8 17 8 24 0 100 5 10
    240606141850_br0i GAGTCTCAGTCCCCTGGAGGCTACAGAAGAAG(+AAAAAA)GATCCTCTGAGGTCAGGGCAACACATGCAAA NGG 10 8 17 8 24 0 100 5 10
    240606141847_7sab CTATATCCCTGGACCCTAGACAAGAGGACCCG(+AAAAAA)GATCCACCTGTGGGCTTCTAGCATTACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606141846_8vbz TAACTCTGGGTAATGCTAGAAGCCCACAGGTG(+AAAAAA)GATCCGGGTCCTCTTGTCTAGGGTCCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606141843_1jhg CTCTGCCTTCACCTGCAGACACCCTCCCTCCG(+AAAAAA)GTACCCTGGGCTTCTACCCTCCCTAGAATTC NGG 10 8 17 8 24 0 100 5 10
    240606141840_e2o1 GATGGGAATTCTAGGGAGGGTAGAAGCCCAGG(+AAAAAA)GTACCGGAGGGAGGGTGTCTGCAGGTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606141838_lnig CCCGCGCTGGACAGCCCAGCGCCCAATCCGGG(+AAAAAA)GATCCAGAGGGTAGGCCTGCCCTTGCACTTA NGG 10 8 17 8 24 0 100 5 10