Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141836_6vdf TGGTTTAAGTGCAAGGGCAGGCCTACCCTCTG(+AAAAAA)GATCCCCGGATTGGGCGCTGGGCTGTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606141835_bd4b GTTAACTGTTACAGAGGAACTGGCTTTCTGAG(+AAAAAA)GTACCTGGCATCTTGGGACGAGTGAAATCTA NGG 10 8 17 8 24 0 100 5 10
    240606141833_gk4q CTGACTAGATTTCACTCGTCCCAAGATGCCAG(+AAAAAA)GTACCTCAGAAAGCCAGTTCCTCTGTAACAG NGG 10 8 17 8 24 0 100 5 10
    240606141831_n92r GGATCTAGCTGGCCCCTTCCCTCCCCAGCCTG(+AAAAAA)GTACCCCAGGTCCAATGACATCAGTCTGACT NGG 10 8 17 8 24 0 100 5 10
    240606141828_79jz AAAGGAGTCAGACTGATGTCATTGGACCTGGG(+AAAAAA)GTACCAGGCTGGGGAGGGAAGGGGCCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606141827_qbnc ACTGAGAAGAAAAGGTTTTAATAAAGAACCAG(+AAAAAA)GATCCTCCCTTCCCCGAGATGGTGGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606141825_xysl CCAGAACACTGCCACCATCTCGGGGAAGGGAG(+AAAAAA)GATCCTGGTTCTTTATTAAAACCTTTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606141823_x2yl TCCCCTGCTGGGTTATGGTGGCACACACCTTG(+AAAAAA)GATCCCCAAACTGAGGGGCATTTGGATCTCT NGG 10 8 17 8 24 0 100 5 10
    240606141821_vd75 AACTCAGAGATCCAAATGCCCCTCAGTTTGGG(+AAAAAA)GATCCAAGGTGTGTGCCACCATAACCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606141819_kcsr CTCAGACAGCTCCAGTGGATCTACTGAATGGG(+AAAAAA)GATCCCTGGTACTGGTGTCTACTCTGCCAAA NGG 10 8 17 8 24 0 100 5 10