Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140637_qpg6 CATCCTAAACACAGCTTCTCTGTGTGTAAAAG(+AAAAAA)GTACCTGCTATTCCTAAGGGGCTGCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240606140636_e1kh ATGTTGCCTGTGCAGCCCCTTAGGAATAGCAG(+AAAAAA)GTACCTTTTACACACAGAGAAGCTGTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240606140634_9q8y TGAAGACTAACTGCTTCTTTGAAGGATTAAAG(+AAAAAA)GATCCCAGCCTAGAACTCATCTGGAGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606140633_fjzs ATGCTCCTGACTCCAGATGAGTTCTAGGCTGG(+AAAAAA)GATCCTTTAATCCTTCAAAGAAGCAGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240606140629_5fhn CCTCCTGGGAAGAAAACTTTTGGCTCCCCCTG(+AAAAAA)GTACCTCAGCAGCGGCATTGCATAGGATTTC NGG 10 8 17 8 24 0 100 5 10
    240606140628_sax0 CTTTTGAAATCCTATGCAATGCCGCTGCTGAG(+AAAAAA)GTACCAGGGGGAGCCAAAAGTTTTCTTCCCA NGG 10 8 17 8 24 0 100 5 10
    240606140627_kwf0 TCCATTTAAACCCTTTATATTTCAACAGTGAG(+AAAAAA)GATCCTTTCCCATGAAGTATTTTCAGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606140626_g80y CCGTGAGACACTGAAAATACTTCATGGGAAAG(+AAAAAA)GATCCTCACTGTTGAAATATAAAGGGTTTAA NGG 10 8 17 8 24 0 100 5 10
    240606140624_1wf9 TTGGGATGGGAGAGACAATAAATGTTTTAGTG(+AAAAAA)GTACCATCTCTATATTCGTCTTCTTTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606140623_psy2 TCCTCTCTGCAAAGAAGACGAATATAGAGATG(+AAAAAA)GTACCACTAAAACATTTATTGTCTCTCCCAT NGG 10 8 17 8 24 0 100 5 10