Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140530_jq4b AGCCAGATGAAACAGCCCAGTTAGGGGAATGG(+AAAAAA)GATCCACAGGCAGGCAATAGATTCAGGAACT NGG 10 8 17 8 24 0 100 5 10
    240606140528_ojnj CATTTAGTTCCTGAATCTATTGCCTGCCTGTG(+AAAAAA)GATCCCATTCCCCTAACTGGGCTGTTTCATC NGG 10 8 17 8 24 0 100 5 10
    240606140525_5lej GTGCAAGGATTTGGGGGGGGGGGGGCGAGGGG(+AAAAAA)GATCCTGAAACCACCACGGGGAGGAAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606140524_sdxq AGGCCACCTCTTCCTCCCCGTGGTGGTTTCAG(+AAAAAA)GATCCCCCTCGCCCCCCCCCCCCCAAATCCT NGG 10 8 17 8 24 0 100 5 10
    240606140522_rgsv CTGAGGGAGCCTGGGCCCGATGTCCTCCCATG(+AAAAAA)GATCCCTGCTTTTGTGGCCTGCTTCAGTTTC NGG 10 8 17 8 24 0 100 5 10
    240606140520_plby GAAGGGAAACTGAAGCAGGCCACAAAAGCAGG(+AAAAAA)GATCCATGGGAGGACATCGGGCCCAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240606140519_eh99 AAGCACAGAGGGGAGAGGGGCCGAGGAAGTAG(+AAAAAA)GTACCTCTCCCCCCCCTTCCCTGTCACCCTC NGG 10 8 17 8 24 0 100 5 10
    240606140518_0nou AGGGGGAGGGTGACAGGGAAGGGGGGGGAGAG(+AAAAAA)GTACCTACTTCCTCGGCCCCTCTCCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606140516_bzsu CAGTATGCAGTATGTGACTTCCAGCACACCAG(+AAAAAA)GTACCCTGCCAGGCACCTTACTTTTGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606140514_012b CTGAGTGCAGCAAAAGTAAGGTGCCTGGCAGG(+AAAAAA)GTACCTGGTGTGCTGGAAGTCACATACTGCA NGG 10 8 17 8 24 0 100 5 10