Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140424_g1id ATGAAATGGACAAATTTCTAGACAGATACCAG(+AAAAAA)GTACCAAAGTTAAATCAGGATCAAGTTAACG NGG 10 8 17 8 24 0 100 5 10
    240606140423_htp8 TAGATCGTTAACTTGATCCTGATTTAACTTTG(+AAAAAA)GTACCTGGTATCTGTCTAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606140421_165v GGTGTCAGCATTTGGAGGCTGATTATGGGATG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606140419_m3xy GGATGGACCATGTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCATCCCATAATCAGCCTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606140417_if2n TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+AAAAAA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606140415_go3t TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AAAAAA)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606140413_3pbx AGGAAAGTGATCCAGAGACTGCCATATCTGGG(+AAAAAA)GATCCATCCTGTATACAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606140412_dnyc AGTGTCTGGGTTTGGTGACTGTATACAGGATG(+AAAAAA)GATCCCCAGATATGGCAGTCTCTGGATCACT NGG 10 8 17 8 24 0 100 5 10
    240606140409_gpj3 TTCATGTATGTGTATGTATGTGTATGTCCCTG(+AAAAAA)GTACCCCTAGAGGCTGAAAAAAGGTGTCACA NGG 10 8 17 8 24 0 100 5 10
    240606140407_wd9b GAGAATGTGACACCTTTTTTCAGCCTCTAGGG(+AAAAAA)GTACCAGGGACATACACATACATACACATAC NGG 10 8 17 8 24 0 100 5 10