Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140406_qdjt GGCAGTGGAATACTGCTACAGGATTGAGATGG(+AAAAAA)GATCCTGGAACAATTTGGTGGAACATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606140404_fab3 TAGTGTTCTTATGTTCCACCAAATTGTTCCAG(+AAAAAA)GATCCCATCTCAATCCTGTAGCAGTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240606140403_ou0j ATGCCAAATGTTTTTTTGATACATATTTGTTG(+AAAAAA)GTACCATGCCTAAAATATACAATGAAGCGAC NGG 10 8 17 8 24 0 100 5 10
    240606140401_q345 GGAATGTCGCTTCATTGTATATTTTAGGCATG(+AAAAAA)GTACCAACAAATATGTATCAAAAAAACATTT NGG 10 8 17 8 24 0 100 5 10
    240606140400_wfz5 AGGAAAACAGAACACGTAAAAGTGTATTACAG(+AAAAAA)GATCCAGAAAGGAAGAAAACAACTTTGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606140359_ezuo AGCAAGACCCAAAGTTGTTTTCTTCCTTTCTG(+AAAAAA)GATCCTGTAATACACTTTTACGTGTTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240606140358_liir CCAGAGTCTAAGGGGATTCGATCCTGTGACAG(+AAAAAA)GTACCTGGAGGTACACTTCACAGAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606140356_1knk GATGCTTCTCTTTCTGTGAAGTGTACCTCCAG(+AAAAAA)GTACCTGTCACAGGATCGAATCCCCTTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606140355_47ri CTAACATACTTCCTTTGAGTGACATGAAATTG(+AAAAAA)GTACCGTTGCTTGTGATGGCGGAGACGTAGT NGG 10 8 17 8 24 0 100 5 10
    240606140353_g8ac ACCTCACTACGTCTCCGCCATCACAAGCAACG(+AAAAAA)GTACCAATTTCATGTCACTCAAAGGAAGTAT NGG 10 8 17 8 24 0 100 5 10