Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140303_3ssp GAGGTGGGAAATGGCCAGAAATCAAGCTGAAG(+AAAAAA)GATCCACCTCCATGTAACCACAGCGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240606140301_6enh GTGTTTCCTGTCGCTGTGGTTACATGGAGGTG(+AAAAAA)GATCCTTCAGCTTGATTTCTGGCCATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606140300_em25 ATCATCACCTTACAGATGTGCCACGCGAAGCG(+AAAAAA)GATCCATTCACCAATGCCTGTGTTCTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240606140259_ggmd TTCTGGTCACAGAACACAGGCATTGGTGAATG(+AAAAAA)GATCCGCTTCGCGTGGCACATCTGTAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606140257_ym49 AGGGTACTTGTGGAGGCCAGAAGAGGGTATTG(+AAAAAA)GATCCTCTGGGGCTGGAGTTATGGGCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606140255_gkph GCTTCCAGCTGCCCATAACTCCAGCCCCAGAG(+AAAAAA)GATCCAATACCCTCTTCTGGCCTCCACAAGT NGG 10 8 17 8 24 0 100 5 10
    240606140254_8eo4 TTTGGAGCCTTAGTATGTTTATTTTGTGGGAG(+AAAAAA)GATCCGCACGCACACAGCTCACTGGAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606140252_qf64 GTCATCTCCCTCCAGTGAGCTGTGTGCGTGCG(+AAAAAA)GATCCTCCCACAAAATAAACATACTAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606140250_5dfq GGTTTGGTTCTTTCTTAGTACTCACAGTGGGG(+AAAAAA)GATCCTGTGGCTCTGATGCTGTCTTCTGACC NGG 10 8 17 8 24 0 100 5 10
    240606140248_mw24 ATGCAGGTCAGAAGACAGCATCAGAGCCACAG(+AAAAAA)GATCCCCCACTGTGAGTACTAAGAAAGAACC NGG 10 8 17 8 24 0 100 5 10