Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140159_vv7s TGACCACGGCACCTTCTGACCCGCTCATCCTG(+AAAAAA)GATCCTGTGGGTTTGGGGTGCGTGGGAACGC NGG 10 8 17 8 24 0 100 5 10
    240606140157_6fju GAAGTGCGTTCCCACGCACCCCAAACCCACAG(+AAAAAA)GATCCAGGATGAGCGGGTCAGAAGGTGCCGT NGG 10 8 17 8 24 0 100 5 10
    240606140156_usa8 AGAAATGGTACAATGTCAGATATCTCAGGGAG(+AAAAAA)GTACCCTGTGGATCTGTATTCCAACGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240606140154_z0mx TCAGGACTCCCGTTGGAATACAGATCCACAGG(+AAAAAA)GTACCTCCCTGAGATATCTGACATTGTACCA NGG 10 8 17 8 24 0 100 5 10
    240606140152_fda6 AGGGCTTGTTGAGTAGGCATCTGGAATGACCG(+AAAAAA)GATCCGGGCATCAGGCCGCTGATGGGCATAG NGG 10 8 17 8 24 0 100 5 10
    240606140150_zmq4 CCACTCTATGCCCATCAGCGGCCTGATGCCCG(+AAAAAA)GATCCGGTCATTCCAGATGCCTACTCAACAA NGG 10 8 17 8 24 0 100 5 10
    240606140148_ibhh GCTACCCAGAGGCCTTTCGTCTCCCTGTTCTG(+AAAAAA)GTACCCAGCTTCCCAATCAGCAGCCAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606140147_w93n TGTTGAGCTCTGGCTGCTGATTGGGAAGCTGG(+AAAAAA)GTACCAGAACAGGGAGACGAAAGGCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606140145_0njz CAGTGTGGATCTGCGGGTTGTCACTAGTCCAG(+AAAAAA)GTACCGAGGACAGACGTTATAGAGTGACACT NGG 10 8 17 8 24 0 100 5 10
    240606140143_dlij TGGACAGTGTCACTCTATAACGTCTGTCCTCG(+AAAAAA)GTACCTGGACTAGTGACAACCCGCAGATCCA NGG 10 8 17 8 24 0 100 5 10