Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140216_s8po TTTAAACCTTTCAAAACAAAATTCCTATCCAG(+AAAAAA)GATCCCATCCAGAGTCATCACCTGCCACTCA NGG 10 8 17 8 24 0 100 5 10
    240606140215_c3ed CTTGTTGAGTGGCAGGTGATGACTCTGGATGG(+AAAAAA)GATCCTGGATAGGAATTTTGTTTTGAAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606140213_qjnf GGGAGGCCATGCCCGCCACCCCTCTTGTTCTG(+AAAAAA)GATCCCTGCTCCCCTCTGGGTGTGTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606140212_yiid CACACACACCCACACACCCAGAGGGGAGCAGG(+AAAAAA)GATCCAGAACAAGAGGGGTGGCGGGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240606140210_y701 AGGCAATTAGAAAAATTATCCAAATAAATTCG(+AAAAAA)GTACCTAGGAAAAAGAAAATAATGTTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606140209_ued1 GAGATAAGGAAACATTATTTTCTTTTTCCTAG(+AAAAAA)GTACCGAATTTATTTGGATAATTTTTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240606140207_w7if GTCACCAGTCACTGGGTCTAGGAGATGTCTTG(+AAAAAA)GTACCCGTCTGTCTGACATTGACAATGACCT NGG 10 8 17 8 24 0 100 5 10
    240606140206_i7aw CTTTCAGGTCATTGTCAATGTCAGACAGACGG(+AAAAAA)GTACCAAGACATCTCCTAGACCCAGTGACTG NGG 10 8 17 8 24 0 100 5 10
    240606140204_rx8t GCAAACAGCAAGAGGCTTTATTGGGAACACGG(+AAAAAA)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240606140201_d29q GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+AAAAAA)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10