Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140232_k4fc CTATTAGGAGAGTAGATGTTAATGTGTTTTGG(+AAAAAA)GTACCCATCTTTGCAACATGTCTGATCCACG NGG 10 8 17 8 24 0 100 5 10
    240606140231_tlmq TGGCACGTGGATCAGACATGTTGCAAAGATGG(+AAAAAA)GTACCCAAAACACATTAACATCTACTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606140229_pxia CTATTAGGAGAGTAGATGTTAATGTGTTTTGG(+AAAAAA)GTACCCATCTTTGCAACATGTCTGATCCACG NGG 10 8 17 8 24 0 100 5 10
    240606140228_o08x TGGCACGTGGATCAGACATGTTGCAAAGATGG(+AAAAAA)GTACCCAAAACACATTAACATCTACTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606140226_dble CTATTAGGAGAATAGATGTTAATGTGTTTTGG(+AAAAAA)GTACCCATCTTTGCAACATGTCTGATCCAAG NGG 10 8 17 8 24 0 100 5 10
    240606140225_nhjb TGGCACTTGGATCAGACATGTTGCAAAGATGG(+AAAAAA)GTACCCAAAACACATTAACATCTATTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606140223_6hvj CTCAGATCCAGTCCACGGGTCCAAGGATGCTG(+AAAAAA)GATCCAAGGACTTCTCTTCCACAGTGGCCGG NGG 10 8 17 8 24 0 100 5 10
    240606140222_avwn GTGGGCCGGCCACTGTGGAAGAGAAGTCCTTG(+AAAAAA)GATCCAGCATCCTTGGACCCGTGGACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606140220_tioy CTGTTCTTGCCCCCGTCACATTCTGGAAAGGG(+AAAAAA)GTACCCAGTGATCTCTGTGACGCCTCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606140218_ofoi GAGGAGTCTGGAGGCGTCACAGAGATCACTGG(+AAAAAA)GTACCCCTTTCCAGAATGTGACGGGGGCAAG NGG 10 8 17 8 24 0 100 5 10