Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140335_6yzs GAATACCACATGCGTTTTTGTCTAATGCTTAG(+AAAAAA)GTACCAAAATGTTAGCATCAGTATTCACTAT NGG 10 8 17 8 24 0 100 5 10
    240606140334_ue92 GCTCTATAGTGAATACTGATGCTAACATTTTG(+AAAAAA)GTACCTAAGCATTAGACAAAAACGCATGTGG NGG 10 8 17 8 24 0 100 5 10
    240606140332_c3kr TGGTATCTGGAAGTCTGTGCACAAGATCTGAG(+AAAAAA)GTACCATTGGGTGGGCTGAGATCTTTAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606140330_tsyn TGGGACACTTAAAGATCTCAGCCCACCCAATG(+AAAAAA)GTACCTCAGATCTTGTGCACAGACTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606140329_3y8q GAGAGAGAGAGAGAGAATATACAGGTACATGG(+AAAAAA)GTACCGATAGAAGTCAGAGACATTGACCTCT NGG 10 8 17 8 24 0 100 5 10
    240606140328_mfhx TCCAGAGAGGTCAATGTCTCTGACTTCTATCG(+AAAAAA)GTACCCATGTACCTGTATATTCTCTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606140326_l1ur ATAGCCTCAAGACAGATCTACATTTTAATGAG(+AAAAAA)GTACCTAAAGTCCTGGAGGCTCAGCCAATAA NGG 10 8 17 8 24 0 100 5 10
    240606140325_qsr0 TGAGTTTATTGGCTGAGCCTCCAGGACTTTAG(+AAAAAA)GTACCTCATTAAAATGTAGATCTGTCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606140324_0ntr TAGGCGCTCGAGAGTTGAGTTAACCCGGTGTG(+AAAAAA)GATCCCGGGCGGAGTCGAGAGCCAGAAGCCG NGG 10 8 17 8 24 0 100 5 10
    240606140321_lucu GCAGCCGGCTTCTGGCTCTCGACTCCGCCCGG(+AAAAAA)GATCCACACCGGGTTAACTCAACTCTCGAGC NGG 10 8 17 8 24 0 100 5 10