Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140319_vy7t TTTTAGCTACATGCACCCATTCACCTTTGGGG(+AAAAAA)GATCCCAACATGTGCATAGTTTTTCAGGTCG NGG 10 8 17 8 24 0 100 5 10
    240606140317_m0sb TAGAACGACCTGAAAAACTATGCACATGTTGG(+AAAAAA)GATCCCCCAAAGGTGAATGGGTGCATGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606140315_rmkx AACCCTTTCTGCAGTTCATATCGCTGTTAGGG(+AAAAAA)GATCCCGATGGGAAAAGAGCCGCGTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606140313_ezl6 CGCTTGCCTGAACGCGGCTCTTTTCCCATCGG(+AAAAAA)GATCCCCTAACAGCGATATGAACTGCAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606140312_nb3g CTGAGAACTCGCTAGGCAAAGAGAAGCTCTTG(+AAAAAA)GATCCTGGCACTCACCTTAAGTCTTTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606140310_rx0e ACAAGTCTACAAAGACTTAAGGTGAGTGCCAG(+AAAAAA)GATCCAAGAGCTTCTCTTTGCCTAGCGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606140308_zu4f TCTTAGGCCACTGTGGCAAGAACTAGGAAGGG(+AAAAAA)GATCCACATCAGAATGCCCTAGCTGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606140307_fm8y GAGATCCCTGCCAGCTAGGGCATTCTGATGTG(+AAAAAA)GATCCCCTTCCTAGTTCTTGCCACAGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240606140305_zp4h AGTTTAATTGCAGAACTGGACCATATCTTTAG(+AAAAAA)GATCCTGTAGGGGAGACAGACATGAATCAAA NGG 10 8 17 8 24 0 100 5 10
    240606140304_ngyt GGTGGTTTGATTCATGTCTGTCTCCCCTACAG(+AAAAAA)GATCCTAAAGATATGGTCCAGTTCTGCAATT NGG 10 8 17 8 24 0 100 5 10